How can I set micropipette volume with decimal separators, for example 109.5 in p200?












2












$begingroup$


P200 ⌸



And how can I set:




  • 150.1 in p200

  • 871.3 in p1000

  • 840.5 in p1000?






molecular-biology







share|improve this question











$endgroup$












  • $begingroup$
    What is the brand and model of your pipet?
    $endgroup$
    – alec_djinn
    Jan 17 at 6:51
















2












$begingroup$


P200 ⌸



And how can I set:




  • 150.1 in p200

  • 871.3 in p1000

  • 840.5 in p1000?






molecular-biology







share|improve this question











$endgroup$












  • $begingroup$
    What is the brand and model of your pipet?
    $endgroup$
    – alec_djinn
    Jan 17 at 6:51














2












2








2





$begingroup$


P200 ⌸



And how can I set:




  • 150.1 in p200

  • 871.3 in p1000

  • 840.5 in p1000?






molecular-biology







share|improve this question











$endgroup$




P200 ⌸



And how can I set:




  • 150.1 in p200

  • 871.3 in p1000

  • 840.5 in p1000?






molecular-biology




molecular-biology






share|improve this question















share|improve this question













share|improve this question




share|improve this question








edited Jan 15 at 9:24









Laurel

1854




1854










asked Jan 14 at 18:09









SaraSara

141




141












  • $begingroup$
    What is the brand and model of your pipet?
    $endgroup$
    – alec_djinn
    Jan 17 at 6:51


















  • $begingroup$
    What is the brand and model of your pipet?
    $endgroup$
    – alec_djinn
    Jan 17 at 6:51
















$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51




$begingroup$
What is the brand and model of your pipet?
$endgroup$
– alec_djinn
Jan 17 at 6:51










3 Answers
3






active

oldest

votes


















6












$begingroup$

It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.






share|improve this answer









$endgroup$









  • 2




    $begingroup$
    I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
    $endgroup$
    – CKM
    Jan 14 at 21:10



















2












$begingroup$

I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.






share|improve this answer









$endgroup$





















    0












    $begingroup$

    For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.






    share|improve this answer











    $endgroup$













    • $begingroup$
      Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
      $endgroup$
      – swbarnes2
      Jan 14 at 23:10










    • $begingroup$
      Interesting. Thanks for sharing I didn't know that.
      $endgroup$
      – Cell
      Jan 15 at 1:53











    Your Answer





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    3 Answers
    3






    active

    oldest

    votes








    3 Answers
    3






    active

    oldest

    votes









    active

    oldest

    votes






    active

    oldest

    votes









    6












    $begingroup$

    It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.






    share|improve this answer









    $endgroup$









    • 2




      $begingroup$
      I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
      $endgroup$
      – CKM
      Jan 14 at 21:10
















    6












    $begingroup$

    It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.






    share|improve this answer









    $endgroup$









    • 2




      $begingroup$
      I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
      $endgroup$
      – CKM
      Jan 14 at 21:10














    6












    6








    6





    $begingroup$

    It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.






    share|improve this answer









    $endgroup$



    It doesn't make sense to set volumes of decimals of microliters with a P200, since usually the accuracy of these pipettes is in the order of a few microliters. So even if you set your pipette at 190 uL, you may still dispense a volume ranging from 187 to 193 uL. For a P1000, due to higher volumes the inaccuracy is of course higher, usually +/- 10 uL or more. Some brands are more accurate than others, like Eppendorf or Finnpipette. I suggest you check the specs of your pipettes and see how much accuracy you can achieve.







    share|improve this answer












    share|improve this answer



    share|improve this answer










    answered Jan 14 at 19:06









    betelgeusebetelgeuse

    1062




    1062








    • 2




      $begingroup$
      I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
      $endgroup$
      – CKM
      Jan 14 at 21:10














    • 2




      $begingroup$
      I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
      $endgroup$
      – CKM
      Jan 14 at 21:10








    2




    2




    $begingroup$
    I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
    $endgroup$
    – CKM
    Jan 14 at 21:10




    $begingroup$
    I think a fair take-home message here is you shouldn't, and with many brands you often can't. Rainin and Eppendorf P1000's measure to four places from 0100 to 1000, for example. The best bet is to use two different pipets, and use them in that fashion throughout the process to maintain consistency.
    $endgroup$
    – CKM
    Jan 14 at 21:10











    2












    $begingroup$

    I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.






    share|improve this answer









    $endgroup$


















      2












      $begingroup$

      I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.






      share|improve this answer









      $endgroup$
















        2












        2








        2





        $begingroup$

        I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.






        share|improve this answer









        $endgroup$



        I would always use multiple pipetters in cases like that, but really the answer is that no pipetter is going to give you 4 sig figs of accuracy. It's just not possible with the technology.







        share|improve this answer












        share|improve this answer



        share|improve this answer










        answered Jan 15 at 16:56









        swbarnes2swbarnes2

        3,555811




        3,555811























            0












            $begingroup$

            For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.






            share|improve this answer











            $endgroup$













            • $begingroup$
              Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
              $endgroup$
              – swbarnes2
              Jan 14 at 23:10










            • $begingroup$
              Interesting. Thanks for sharing I didn't know that.
              $endgroup$
              – Cell
              Jan 15 at 1:53
















            0












            $begingroup$

            For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.






            share|improve this answer











            $endgroup$













            • $begingroup$
              Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
              $endgroup$
              – swbarnes2
              Jan 14 at 23:10










            • $begingroup$
              Interesting. Thanks for sharing I didn't know that.
              $endgroup$
              – Cell
              Jan 15 at 1:53














            0












            0








            0





            $begingroup$

            For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.






            share|improve this answer











            $endgroup$



            For p200 the smallest unit is the tenth of a ul (0.1) but the intervals go by 0.2 so to set 109.5 (assuming your pipette can be adjusted between intervals) you need to set the pipette so the pointer is equidistant from the 109.4 and 109.6 mark. For the p1000 the smallest unit is 1 ul so you cannot set it to 871.3.







            share|improve this answer














            share|improve this answer



            share|improve this answer








            edited Jan 15 at 1:55

























            answered Jan 14 at 21:17









            CellCell

            385116




            385116












            • $begingroup$
              Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
              $endgroup$
              – swbarnes2
              Jan 14 at 23:10










            • $begingroup$
              Interesting. Thanks for sharing I didn't know that.
              $endgroup$
              – Cell
              Jan 15 at 1:53


















            • $begingroup$
              Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
              $endgroup$
              – swbarnes2
              Jan 14 at 23:10










            • $begingroup$
              Interesting. Thanks for sharing I didn't know that.
              $endgroup$
              – Cell
              Jan 15 at 1:53
















            $begingroup$
            Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
            $endgroup$
            – swbarnes2
            Jan 14 at 23:10




            $begingroup$
            Some brands of pipeters "click" at each allowed increment, so you won't be able to go between them.
            $endgroup$
            – swbarnes2
            Jan 14 at 23:10












            $begingroup$
            Interesting. Thanks for sharing I didn't know that.
            $endgroup$
            – Cell
            Jan 15 at 1:53




            $begingroup$
            Interesting. Thanks for sharing I didn't know that.
            $endgroup$
            – Cell
            Jan 15 at 1:53


















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